Search results for "molecular detection"

showing 6 items of 6 documents

Statements from the Taormina expert meeting on occult hepatitis B virus infection

2008

Giovanni Raimondo*, Jean-Pierre Allain, Maurizia R. Brunetto, Marie-Annick Buendia, Ding-Shinn Chen, Massimo Colombo, Antonio Craxi, Francesco Donato, Carlo Ferrari, Giovanni B. Gaeta, Wolfram H. Gerlich, Massimo Levrero, Stephen Locarnini, Thomas Michalak, Mario U. Mondelli, Jean-Michel Pawlotsky, Teresa Pollicino, Daniele Prati, Massimo Puoti, Didier Samuel, Daniel Shouval, Antonina Smedile, Giovanni Squadrito, Christian Trepo, Erica Villa, Hans Will, Alessandro R. Zanetti, Fabien Zoulim

HBV; guidelinesmedia_common.quotation_subjectviral hepatitisOccult hepatitis B virus infection OBISeropositive OBI and Seronegative OBImedicine.disease_causeOccult hepatitis B virus; molecular detection of HBV DNA; viral hepatitis; HBV transmissionOccult hepatitis B virusViral geneticsHBVmedicineguidelinesmedia_commonHepatitis B virusOccult hepatitis B virus infection OBI; Seropositive OBI and Seronegative OBI; False OBI; Clinical impact of OBI; Diagnosis of OBI and epidemiological aspectsHepatologymolecular detection of HBV DNAFalse OBIClinical impact of OBIArtOccult hepatitis B infectionOccultVirologyDiagnosis of OBI and epidemiological aspectsHBV transmissionHumanities
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Nation-wide study of the occurrence of Listeria monocytogenes in French soils using culture-based and molecular detection methods

2013

Identifiant HAL : hal-01120618; International audience; Soil is a potential reservoir of human pathogens and a possible source of contamination of animals, crops and water. In order to study the distribution of Listeria monocytogenes in French soils, a real-time PCR TaqMan assay targeting the phosphoribosylpyrophosphate synthetase (prs) gene of L. monocytogenes was developed for the specific detection and quantification of this bacterium within a collection of 1315 soil DNAs originated from the French Soil Quality Monitoring Network. The prs real-time PCR TaqMan assay was specific for L. monocytogenes and could quantify accurately down to 104L. monocytogenes per gram of dry soil. Among the …

Microbiology (medical)DNA BacterialVeterinary medicineColony Count MicrobialFrench soil monitoring networkmedicine.disease_causeReal-Time Polymerase Chain ReactionMicrobiologyPasturecomplex mixturesTaqMan type probeMicrobiologyCulture-based detection03 medical and health sciencesListeria monocytogenesmedicineTaqMan[ SDU.ENVI ] Sciences of the Universe [physics]/Continental interfaces environmentRibose-Phosphate PyrophosphokinaseSerotyping[SDU.ENVI]Sciences of the Universe [physics]/Continental interfaces environmentMolecular BiologyMolecular detectionSoil Microbiology030304 developmental biologyGramDNA Primers2. Zero hunger0303 health sciencesgeography[ SDE.BE ] Environmental Sciences/Biodiversity and EcologyBacteriological Techniquesgeography.geographical_feature_categorybiology030306 microbiologyContaminationbiology.organism_classificationSoil qualityListeria monocytogenesBacterial Typing TechniquesSoil waterFrance[SDE.BE]Environmental Sciences/Biodiversity and EcologyBacteriaReal-time PCR
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A molecular method to assess Phytophthora diversity in environmental samples

2012

Current molecular detection methods for the genus Phytophthora are specific to a few key species rather than the whole genus and this is a recognized weakness of protocols for ecological studies and international plant health legislation. In the present study a molecular approach was developed to detect Phytophthora species in soil and water samples using novel sets of genus-specific primers designed against the internal transcribed spacer (ITS) regions. Two different rDNA primer sets were tested: one assay amplified a long product including the ITS1, 5.8S and ITS2 regions (LP) and the other a shorter product including the ITS1 only (SP). Both assays specifically amplified products from Phy…

Microbiology (medical)PhytophthoraGenus-specific primersRange (biology)Molecular Sequence DataBiodiversityMetagenomic analysesMicrobiologyNatural ecosystemsMicrobial ecologyBotanyDNA Ribosomal SpacerEnvironmental MicrobiologyPythiumInternal transcribed spacerMolecular BiologyMolecular detectionPhytophthora sppDNA PrimersPhylotypebiologyPhytophthora spp.; Molecular detection; Metagenomic analyses; Genus-specific primers; Natural ecosystemsfungifood and beveragesBiodiversitySequence Analysis DNAbiology.organism_classificationClassificationScotlandMetagenomicsPhytophthora
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TOOLS TO DETECT, IDENTIFY AND MONITOR PHYTOPHTHORA SPECIES IN NATURAL ECOSYSTEM

2007

Phytophthoramolecular detectionecologytarget genesPhytophthora; natural ecosystems; ecology; molecular detection; target genesnatural ecosystems
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Molecular detection and monitoring of fungal plant symbionts

2003

International audience

[SDV] Life Sciences [q-bio][SDV]Life Sciences [q-bio]Molecular detectionComputingMilieux_MISCELLANEOUSfungal plant symbionts
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Identification of functional markers of mycorrhiza efficiency in vineyard

2021

Arbuscular mycorrhizal fungi form a mutualistic symbiotic association with more than 80% ofland plants, including vines. Advantages of this mutualistic symbiosis are countless. Rootcolonisation by mycorrhizal fungi is mainly evaluated through staining and microscopicobservation. However those technics do not allow to differentiate different mycorrhizal fungi.In order to overcome those limits other tools, like antibodies, isoenzymes and molecular biologytechnics, can be used. Nevertheless, all of these methods “only” allow detection of the presenceof mycorrhizal fungi, whereas no information is provided on the proper mycorrhizal symbiosisfunctioning, and therefore on the benefits provided to…

[SDV] Life Sciences [q-bio]molecular detection kitarbuscular mycorrhizavineyardfunctional markers
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